The high-pressure liquid chromatographic method is of practical importance for the separation of mixtures composed of many components only if the reproducibility of the method is guaranteed.
On the basis of experiments the following problems are discussed: Retention time as a function of the water content of the eluents, apparative requirements for quantitative analysis, reduction of column efficiency by connecting tubes, quantitative definition of a "good" separation column, applicability of reversed phases and that of exclusion chromatography in the field of mineral oil analysis.